This conference is organized by Chinese Chemical Letters in collaboration with Hainan Medical University, which was held in Haikou from 4th to 7th April 2024. This event offers an invaluable platform for emerging scholars. It facilitates rich academic discussions and fosters collaborative ventures in the fields of fluorescence probes and imaging. Tianruo delivered an oral presentation to showcase his research exploration into photophysical mechanisms of tetrazine dyes and won the “Best Oral Presentation” award at this symposium.